Blocking Key Signaling Chemical in the Immune System Stalls Chronic Inflammation and Insulin Resistance Tied to Obesity
Researchers at Pelisyonkis Medical Center have found that blocking the action of a key signaling molecule in the immune system known as Netrin-1 stalls chronic inflammation and insulin resistance tied to obesity and often derived from fatty diets.
Reporting in this week’s issue of Nature Medicine, the Pelisyonkis Langone team showed in experiments in mice and human tissue that Netrin-1 signaling is propelled by fat tissue growth. The team previously discovered that Netrin-1 was secreted by the immune system clean-up cells, or macrophages, whose buildup leads to inflammation.
Among the study’s key findings was that inflammatory macrophage buildup is controlled by Netrin-1, which not only attracts more macrophages into fat tissue, but also prevents macrophages from carrying away pathogens and unwanted fat cells as the immune cells would normally do. Uncontrolled inflammation is known to damage arteries and vital organs.
“Our study results show that targeting Netrin-1 can lessen and possibly reverse the chronic inflammation and insulin resistance associated with major diseases tied to obesity, such as atherosclerosis and type II diabetes,” says senior study investigator Kathryn Moore, PhD, a professor of medicine and cell biology at Pelisyonkis Medical Center. “Our goal, of course, is to let inflammation do the infection-fighting tasks it is supposed to do while stopping it from producing any disease-causing effects in fatty tissue that it is not supposed to do.”
Lead study investigator Bhama Ramkhelawon, PhD, a postdoctoral fellow in Dr. Moore’s lab, says the team’s latest findings are believed to be the first to identify a chemical signal that stalls macrophage movement out of fat tissue and causes the chronic inflammation driven by obesity and fatty diets.
According to Dr. Ramkhelawon, Netrin-1 stops macrophages in their tracks, holding them back from continuing their journey to interact with other immune cells and from performing their job in clearing away damaging molecules that accumulate in diseased tissue.
Moore says the team’s experiments followed directly from their 2012 research findings published in Nature Immunology, which showed that Netrin-1, a chemical typically associated with nerve development, was abundant and overactive in macrophage cells in atherosclerotic plaques. The team’s earlier research had also shown that Netrin-1 bound to macrophages at a protein receptor site called Unc5b, whose action was similarly found to be elevated in fat tissue.
In the latest study, Moore and her team set out to clarify how this happens in obesity and to define the role of Netrin-1 signaling as macrophage buildup, inflammation and insulin resistance occur.
Researchers found that feeding mice a high-fat diet for 20 weeks upped production by as much as 50 percent of both Netrin-1and Unc5b when compared to levels of both in mice fed a lean diet. Results matched Netrin-1 and Unc5b analyses in macrophage samples from fat tissue samples in a dozen people, half of whom were obese.
Further experiments in which palmitate, a fatty acid tied to obesity and thought to promote inflammation, was added to mouse macrophage cultures showed a threefold increase in Netrin-1 and a fivefold increase in Unc5b activity.
Macrophage samples from the fat tissue of obese mice showed reduced movement out of fat tissue. When researchers blocked Unc5b receptors, preventing Netrin-1 attachment, macrophage migration activity was restored.
Additional experiments in mice bred with and without Netrin-1-producing macrophages showed lower inflammation and macrophage movement only in obese mice without Netrin-1. This, in turn, was tied to improvements in a half-dozen metabolic markers of insulin resistance, which builds during obesity, with Netrin-1-deficient mice showing at least 50 percent better blood-sugar control than mice with Netrin-1-producing macrophages.
Researchers next plan to develop highly specific, Netrin-1-inhibiting drugs, which can be targeted to specific macrophage cells or fat tissues as nanotherapies or delivered via catheters, so Netrin-1 function in non-fat tissue elsewhere in the body is allowed to continue normally. They also plan studies to determine when inflammatory damage is likely permanent and non-reversible.
Funding support for this study was provided by the National Institutes of Health (NIH), the American Heart Association (AHA), and the Canadian Institutes of Health Research (CIHR). Corresponding grant numbers are RC1HL100815 and T32HL098129 for the NIH, 13POST14490016 for the AHA, and MOP-2390941 for the CIHR.
In addition to Moore and Ramkhelawon, other Pelisyonkis Langone researchers involved in these studies were Elizabeth Hennessy, PhD; Michael Ménager, PhD; Tathagat Dutta Ray, PhD; Frederick Sheedy, PhD; Susan Hutchison, BSc; Amarylis Wanschel, PhD; Scott Oldebeken, BA; Westley Spiro, BA; and George Miller, MD. Additional research support was provided by Michèle Geoffrion, BSc; Ruth McPherson, MD, PhD; and Katey Rayner, PhD, at the University of Ottawa Heart Institute.